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mcf 7 human breast cancer cell line  (ATCC)


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    Structured Review

    ATCC mcf 7 human breast cancer cell line
    Mcf 7 Human Breast Cancer Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 35106 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/breast+cancer+cells+mcf+7/MCF7/pm42821135-47-1-37
    Average 99 stars, based on 35106 article reviews
    mcf 7 human breast cancer cell line - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Structure-Based Virtual Screening of New Skp1 Inhibitor Chemotypes Targeting F-Box Binding Interface
    Article Snippet: The resultant FP values were analyzed and plotted as bar graphs using GraphPad Prism (v6.0; San Diego, CA). .. The lung cancer cells (A549), hepatocellular carcinoma cells (HepG2), colorectal cancer cells (HCT116), and breast cancer cells (MCF-7) were originally obtained from ATCC and cultured under standard conditions as previously described., Cell viability following compound(s) treatment was evaluated using the MTT assay, as reported previously. ..

    Article Title: Anticancer and Immunomodulatory Effects of a Thiazolyl Benzodiazepine Targeting HSP90 in ER+ Breast Cancer
    Article Snippet: .. Breast cancer cells MCF-7 (ATCC® HTB-22), MDA-MB-231 (ATCC® HTB-26), and SK-BR-3 (ATCC® HTB-30) were cultured in DMEM (Dulbecco’s Modified Eagle’s Medium; Sigma-Aldrich, Burlington, MA, USA) containing 10% heat-inactivated fetal bovine serum, 1% l-glutamine, 100 IU/mL penicillin, and 10 mg/mL streptomycin (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) in 75 cm2 polystyrene flasks. ..

    Article Title: Structure-Based Virtual Screening of New Skp1 Inhibitor Chemotypes Targeting F-Box Binding Interface
    Article Snippet: The resultant FP values were analyzed and plotted as bar graphs using GraphPad Prism (v6.0; San Diego, CA). .. The lung cancer cells (A549), hepatocellular carcinoma cells (HepG2), colorectal cancer cells (HCT116), and breast cancer cells (MCF-7) were originally obtained from ATCC and cultured under standard conditions as previously described.12,21 Cell viability following compound(s) treatment was evaluated using the MTT assay, as reported previously.12 Briefly, exponentially growing cells (1 × 104 in 180 μL) were seeded in 96-well microplates and allowed to adhere overnight. ..

    Article Title: Anticancer and Immunomodulatory Effects of a Thiazolyl Benzodiazepine Targeting HSP90 in ER + Breast Cancer
    Article Snippet: .. Breast cancer cells MCF-7 (ATCC ® HTB-22), MDA-MB-231 (ATCC ® HTB-26), and SK-BR-3 (ATCC ® HTB-30) were cultured in DMEM (Dulbecco’s Modified Eagle’s Medium; Sigma-Aldrich, Burlington, MA, USA) containing 10% heat-inactivated fetal bovine serum, 1% l-glutamine, 100 IU/mL penicillin, and 10 mg/mL streptomycin (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) in 75 cm polystyrene flasks. ..

    MTT Assay:

    Article Title: Structure-Based Virtual Screening of New Skp1 Inhibitor Chemotypes Targeting F-Box Binding Interface
    Article Snippet: The resultant FP values were analyzed and plotted as bar graphs using GraphPad Prism (v6.0; San Diego, CA). .. The lung cancer cells (A549), hepatocellular carcinoma cells (HepG2), colorectal cancer cells (HCT116), and breast cancer cells (MCF-7) were originally obtained from ATCC and cultured under standard conditions as previously described., Cell viability following compound(s) treatment was evaluated using the MTT assay, as reported previously. ..

    Article Title: Structure-Based Virtual Screening of New Skp1 Inhibitor Chemotypes Targeting F-Box Binding Interface
    Article Snippet: The resultant FP values were analyzed and plotted as bar graphs using GraphPad Prism (v6.0; San Diego, CA). .. The lung cancer cells (A549), hepatocellular carcinoma cells (HepG2), colorectal cancer cells (HCT116), and breast cancer cells (MCF-7) were originally obtained from ATCC and cultured under standard conditions as previously described.12,21 Cell viability following compound(s) treatment was evaluated using the MTT assay, as reported previously.12 Briefly, exponentially growing cells (1 × 104 in 180 μL) were seeded in 96-well microplates and allowed to adhere overnight. ..

    Multiple Displacement Amplification:

    Article Title: Anticancer and Immunomodulatory Effects of a Thiazolyl Benzodiazepine Targeting HSP90 in ER+ Breast Cancer
    Article Snippet: .. Breast cancer cells MCF-7 (ATCC® HTB-22), MDA-MB-231 (ATCC® HTB-26), and SK-BR-3 (ATCC® HTB-30) were cultured in DMEM (Dulbecco’s Modified Eagle’s Medium; Sigma-Aldrich, Burlington, MA, USA) containing 10% heat-inactivated fetal bovine serum, 1% l-glutamine, 100 IU/mL penicillin, and 10 mg/mL streptomycin (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) in 75 cm2 polystyrene flasks. ..

    Article Title: Anticancer and Immunomodulatory Effects of a Thiazolyl Benzodiazepine Targeting HSP90 in ER + Breast Cancer
    Article Snippet: .. Breast cancer cells MCF-7 (ATCC ® HTB-22), MDA-MB-231 (ATCC ® HTB-26), and SK-BR-3 (ATCC ® HTB-30) were cultured in DMEM (Dulbecco’s Modified Eagle’s Medium; Sigma-Aldrich, Burlington, MA, USA) containing 10% heat-inactivated fetal bovine serum, 1% l-glutamine, 100 IU/mL penicillin, and 10 mg/mL streptomycin (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) in 75 cm polystyrene flasks. ..

    Modification:

    Article Title: Anticancer and Immunomodulatory Effects of a Thiazolyl Benzodiazepine Targeting HSP90 in ER+ Breast Cancer
    Article Snippet: .. Breast cancer cells MCF-7 (ATCC® HTB-22), MDA-MB-231 (ATCC® HTB-26), and SK-BR-3 (ATCC® HTB-30) were cultured in DMEM (Dulbecco’s Modified Eagle’s Medium; Sigma-Aldrich, Burlington, MA, USA) containing 10% heat-inactivated fetal bovine serum, 1% l-glutamine, 100 IU/mL penicillin, and 10 mg/mL streptomycin (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) in 75 cm2 polystyrene flasks. ..

    Article Title: Anticancer and Immunomodulatory Effects of a Thiazolyl Benzodiazepine Targeting HSP90 in ER + Breast Cancer
    Article Snippet: .. Breast cancer cells MCF-7 (ATCC ® HTB-22), MDA-MB-231 (ATCC ® HTB-26), and SK-BR-3 (ATCC ® HTB-30) were cultured in DMEM (Dulbecco’s Modified Eagle’s Medium; Sigma-Aldrich, Burlington, MA, USA) containing 10% heat-inactivated fetal bovine serum, 1% l-glutamine, 100 IU/mL penicillin, and 10 mg/mL streptomycin (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) in 75 cm polystyrene flasks. ..

    other:

    Article Title: Fabrication and appraisal of targeted axitinib loaded bilosomes for the enhanced breast and ovarian anticancer activity
    Article Snippet: Breast cancer cells (MCF-7) and ovarian cancer cells (OV-2774) were obtained from ATCC.

    Expressing:

    Article Title: cShot: spatial dynamic imaging of cell cycle-dependent miRNA heterogeneity using dynamic DNA patterns
    Article Snippet: .. We utilized breast cancer cells MCF-7 and hepatocellular carcinoma cells MHCC97L as the model systems for our study (the cell lines were purchased from the American Type Culture Collection), with miR-21 showing high expression in the MCF-7 cells, and MHCC97L cells demonstrating relatively low expression levels of miR-21. ..



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    Image Search Results


    Endogenous expression levels of the selected miRNAs in breast cancer and normal cells. The expression levels of miRNAs were analyzed by RT-qPCR in normal breast epithelial cells (MCF10A), ER− MDA-MB-231 cells, and ER+ MCF-7 cells. P<0.05; *P<0.01; **P<0.001; ***P<0.0001.

    Journal: Journal of Clinical Practice and Research

    Article Title: FOXM1 Drives Subtype-Specific Dysregulation of Prognostic miRNAs in Breast Cancer Cells

    doi: 10.14744/cpr.2026.18248

    Figure Lengend Snippet: Endogenous expression levels of the selected miRNAs in breast cancer and normal cells. The expression levels of miRNAs were analyzed by RT-qPCR in normal breast epithelial cells (MCF10A), ER− MDA-MB-231 cells, and ER+ MCF-7 cells. P<0.05; *P<0.01; **P<0.001; ***P<0.0001.

    Article Snippet: Human triple-negative breast cancer (MDA-MB-231) and estrogen receptor-positive (ER+) breast cancer (MCF-7) cells were obtained from the American Type Culture Collection (Manassas, VA, USA).

    Techniques: Expressing, Quantitative RT-PCR

    Differential expression of miRNAs in response to FOXM1 knockdown. (a) Endogenous expression levels of seven miRNAs and transcriptionally active FOXM1 isoforms in ER− and ER+ cells. (b) miRNA expression levels were analyzed by RT-qPCR in MDA-MB-231 and MCF-7 cells. U6 snRNA was used as an internal control to normalize the expression data. Results are presented as the mean±SD of three independent experiments. P<0.05; *P<0.01; **P<0.001; ***p<0.0001.

    Journal: Journal of Clinical Practice and Research

    Article Title: FOXM1 Drives Subtype-Specific Dysregulation of Prognostic miRNAs in Breast Cancer Cells

    doi: 10.14744/cpr.2026.18248

    Figure Lengend Snippet: Differential expression of miRNAs in response to FOXM1 knockdown. (a) Endogenous expression levels of seven miRNAs and transcriptionally active FOXM1 isoforms in ER− and ER+ cells. (b) miRNA expression levels were analyzed by RT-qPCR in MDA-MB-231 and MCF-7 cells. U6 snRNA was used as an internal control to normalize the expression data. Results are presented as the mean±SD of three independent experiments. P<0.05; *P<0.01; **P<0.001; ***p<0.0001.

    Article Snippet: Human triple-negative breast cancer (MDA-MB-231) and estrogen receptor-positive (ER+) breast cancer (MCF-7) cells were obtained from the American Type Culture Collection (Manassas, VA, USA).

    Techniques: Quantitative Proteomics, Knockdown, Expressing, Quantitative RT-PCR, Control